
Professor
Diabetes Center
+1 415 476-4661
Michael McManus, PhD studies biological processes relating to RNA interference pathways in mouse models. This includes the study of small regulatory RNAs of biological significance that help control development in mammals.
Publications
miR-200 deficiency promotes lung cancer metastasis by activating Notch signaling in cancer-associated fibroblasts.
Genes & development
Examining the evidence for extracellular RNA function in mammals.
Nature reviews. Genetics
High-Throughput CRISPR Screening Identifies Genes Involved in Macrophage Viability and Inflammatory Pathways.
Cell reports
miR-29 Sustains B Cell Survival and Controls Terminal Differentiation via Regulation of PI3K Signaling.
Cell reports
Macrophage Exosomes Resolve Atherosclerosis by Regulating Hematopoiesis and Inflammation via MicroRNA Cargo.
Cell reports
Host-Receptor Post-Translational Modifications Refine Staphylococcal Leukocidin Cytotoxicity.
Toxins
Genomic Resolution of DLX-Orchestrated Transcriptional Circuits Driving Development of Forebrain GABAergic Neurons.
Cell reports
High-Complexity shRNA Libraries and PI3 Kinase Inhibition in Cancer: High-Fidelity Synthetic Lethality Predictions.
Cell reports
Far away from the lamppost.
PLoS biology
miR-205 Regulates Basal Cell Identity and Stem Cell Regenerative Potential During Mammary Reconstitution.
Stem cells (Dayton, Ohio)
Publisher Correction: Human CD45 is an F-component-specific receptor for the staphylococcal toxin Panton-Valentine leukocidin.
Nature microbiology
A Genetic Interaction Map of Insulin Production Identifies Mfi as an Inhibitor of Mitochondrial Fission.
Endocrinology
Human CD45 is an F-component-specific receptor for the staphylococcal toxin Panton-Valentine leukocidin.
Nature microbiology
Corrigendum: A systematic comparison reveals substantial differences in chromosomal versus episomal encoding of enhancer activity.
Genome research
Genome-wide CRISPR screen identifies FAM49B as a key regulator of actin dynamics and T cell activation.
Proceedings of the National Academy of Sciences of the United States of America
Unexplored therapeutic opportunities in the human genome.
Nature reviews. Drug discovery
Unexplored therapeutic opportunities in the human genome.
Nature reviews. Drug discovery
The Transcriptionally Permissive Chromatin State of Embryonic Stem Cells Is Acutely Tuned to Translational Output.
Cell stem cell
Dual Strategies for Argonaute2-Mediated Biogenesis of Erythroid miRNAs Underlie Conserved Requirements for Slicing in Mammals.
Molecular cell
Dual gene activation and knockout screen reveals directional dependencies in genetic networks.
Nature biotechnology
CRISPR/Cas-based screening of long non-coding RNAs (lncRNAs) in macrophages with an NF-κB reporter.
The Journal of biological chemistry
Defining epithelial cell dynamics and lineage relationships in the developing lacrimal gland.
Development (Cambridge, England)
miR-205 is a critical regulator of lacrimal gland development.
Developmental biology
MicroRNAs in ectodermal appendages.
Current opinion in genetics & development
A systematic comparison reveals substantial differences in chromosomal versus episomal encoding of enhancer activity.
Genome research
PIM1 kinase inhibition as a targeted therapy against triple-negative breast tumors with elevated MYC expression.
Nature medicine
Biogenesis, delivery, and function of extracellular RNA.
Journal of extracellular vesicles
Choosing the Right Tool for the Job: RNAi, TALEN, or CRISPR.
Molecular cell
Epigenetic and transcriptional determinants of the human breast.
Nature communications
A screen in mice uncovers repression of lipoprotein lipase by microRNA-29a as a mechanism for lipid distribution away from the liver.
Hepatology (Baltimore, Md.)
A high-coverage shRNA screen identifies TMEM129 as an E3 ligase involved in ER-associated protein degradation.
Nature communications
Discovering the complexity of the metazoan transcriptome.
Genome biology
Massively parallel functional annotation of 3' untranslated regions.
Nature biotechnology
Negative regulation of Hif1a expression and TH17 differentiation by the hypoxia-regulated microRNA miR-210.
Nature immunology
Next-generation NAMPT inhibitors identified by sequential high-throughput phenotypic chemical and functional genomic screens.
Chemistry & biology
Partially penetrant postnatal lethality of an epithelial specific MicroRNA in a mouse knockout.
PloS one
The Pitx2:miR-200c/141:noggin pathway regulates Bmp signaling and ameloblast differentiation.
Development (Cambridge, England)
Pervasive transcription of the human genome produces thousands of previously unidentified long intergenic noncoding RNAs.
PLoS genetics
Renewable RNAi.
Nature biotechnology
A systematic mammalian genetic interaction map reveals pathways underlying ricin susceptibility.
Cell
T cell activation induces proteasomal degradation of Argonaute and rapid remodeling of the microRNA repertoire.
The Journal of experimental medicine
IRE1a cleaves select microRNAs during ER stress to derepress translation of proapoptotic Caspase-2.
Science (New York, N.Y.)
Research resource: RNA-Seq reveals unique features of the pancreatic �-cell transcriptome.
Molecular endocrinology (Baltimore, Md.)
Right- and left-loop short shRNAs have distinct and unusual mechanisms of gene silencing.
Nucleic acids research
Widespread RNA 3'-end oligouridylation in mammals.
RNA (New York, N.Y.)
An siRNA screen in pancreatic beta cells reveals a role for Gpr27 in insulin production.
PLoS genetics
The microRNA-processing enzyme Dicer is dispensable for somite segmentation but essential for limb bud positioning.
Developmental biology
Analysis of microRNA knockouts in mice.
Human molecular genetics
Dicer ablation in oligodendrocytes provokes neuronal impairment in mice.
Annals of neurology
Expanded RNA-binding activities of mammalian Argonaute 2.
Nucleic acids research
Dicer is required for proper liver zonation.
The Journal of pathology
Lentivirus production.
Journal of visualized experiments : JoVE
LPS induces KH-type splicing regulatory protein-dependent processing of microRNA-155 precursors in macrophages.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology
Up-regulation of miR-21 by HER2/neu signaling promotes cell invasion.
The Journal of biological chemistry
Disruption of Dicer1 induces dysregulated fetal gene expression and promotes hepatocarcinogenesis.
Gastroenterology
Characterization of the 1-aminocyclopropane-1-carboxylic acid (ACC) oxidase multigene family of Malus domestica Borkh.
Phytochemistry
Residual microRNA expression dictates the extent of inner ear development in conditional Dicer knockout mice.
Developmental biology
Genetic analyses reveal a requirement for Dicer1 in the mouse urogenital tract.
Mammalian genome : official journal of the International Mammalian Genome Society
Sertoli cell Dicer is essential for spermatogenesis in mice.
Developmental biology
Podocyte-specific deletion of dicer alters cytoskeletal dynamics and causes glomerular disease.
Journal of the American Society of Nephrology : JASN
Selective miRNA disruption in T reg cells leads to uncontrolled autoimmunity.
The Journal of experimental medicine
Dicer1 is required for differentiation of the mouse male germline.
Biology of reproduction
Dicer inactivation leads to progressive functional and structural degeneration of the mouse retina.
The Journal of neuroscience : the official journal of the Society for Neuroscience
Conditional loss of Dicer disrupts cellular and tissue morphogenesis in the cortex and hippocampus.
The Journal of neuroscience : the official journal of the Society for Neuroscience
Dicer loss in striatal neurons produces behavioral and neuroanatomical phenotypes in the absence of neurodegeneration.
Proceedings of the National Academy of Sciences of the United States of America
Michael T. McManus: Interrupting biology [interview by Hema Bashyam].
The Journal of experimental medicine
Unintentional miRNA ablation is a risk factor in gene knockout studies: a short report.
PLoS genetics
Behind the scenes of a small RNA gene-silencing pathway.
Human gene therapy
Further examination of abscission zone cells as ethylene target cells in higher plants.
Annals of botany
Essential role for Dicer during skeletal muscle development.
Developmental biology
Complex formation between recombinant ATP sulfurylase and APS reductase of Allium cepa (L.).
FEBS letters
Lentiviral strategies for RNAi knockdown of neuronal genes.
Current protocols in neuroscience
Dicer function is essential for lung epithelium morphogenesis.
Proceedings of the National Academy of Sciences of the United States of America
MicroRNAs and endocrine biology.
The Journal of endocrinology
Slowing down the Ras lane: miRNAs as tumor suppressors?
Science's STKE : signal transduction knowledge environment
TAZ, a transcriptional modulator of mesenchymal stem cell differentiation.
Science (New York, N.Y.)
The RNaseIII enzyme Dicer is required for morphogenesis but not patterning of the vertebrate limb.
Proceedings of the National Academy of Sciences of the United States of America
Small RNAs and immunity.
Immunity
MicroRNA-responsive 'sensor' transgenes uncover Hox-like and other developmentally regulated patterns of vertebrate microRNA expression.
Nature genetics
Cre-lox-regulated conditional RNA interference from transgenes.
Proceedings of the National Academy of Sciences of the United States of America
MicroRNAs and cancer.
Seminars in cancer biology
RNA interference of influenza virus production by directly targeting mRNA for degradation and indirectly inhibiting all viral RNA transcription.
Proceedings of the National Academy of Sciences of the United States of America
A lentivirus-based system to functionally silence genes in primary mammalian cells, stem cells and transgenic mice by RNA interference.
Nature genetics
Small interfering RNA-mediated gene silencing in T lymphocytes.
Journal of immunology (Baltimore, Md. : 1950)
Gene silencing in mammals by small interfering RNAs.
Nature reviews. Genetics
CP110, a cell cycle-dependent CDK substrate, regulates centrosome duplication in human cells.
Developmental cell
Gene silencing using micro-RNA designed hairpins.
RNA (New York, N.Y.)
Identification of candidate mitochondrial RNA editing ligases from Trypanosoma brucei.
RNA (New York, N.Y.)
Processing of polycistronic guide RNAs is associated with RNA editing complexes in Trypanosoma brucei.
The EMBO journal
Trypanosoma brucei guide RNA poly(U) tail formation is stabilized by cognate mRNA.
Molecular and cellular biology
Biochemical methods for analysis of kinetoplastid RNA editing.
Methods (San Diego, Calif.)
Insertional and deletional RNA editing in trypanosome mitochondria.
Nucleic acids symposium series