
Professor
Medicine
+1 415 734-2000
Bruce Conklin, MD is a senior investigator at the Gladstone Institute of Cardiovascular Disease. His research focuses on genes involved in abnormal heart rhythms and heart failure with the goal of developing better, more personalized heart drugs.
Publications
Generation and characterization of a human induced pluripotent stem cell (iPSC) line from a patient with BAG3 P209L myofibrillar myopathy-6.
Stem cell research
Neuronal DNA repair reveals strategies to influence CRISPR editing outcomes.
bioRxiv : the preprint server for biology
Haplotype editing with CRISPR/Cas9 as a therapeutic approach for dominant-negative missense mutations in NEFL.
bioRxiv : the preprint server for biology
Reversal of C9orf72 mutation-induced transcriptional dysregulation and pathology in cultured human neurons by allele-specific excision.
Alzheimer's & Dementia
Programmable epigenome editing by transient delivery of CRISPR epigenome editor ribonucleoproteins.
bioRxiv : the preprint server for biology
Permittivity tensor imaging: modular label-free imaging of 3D dry mass and 3D orientation at high resolution.
Nature methods
Advances and challenges in modeling inherited peripheral neuropathies using iPSCs.
Experimental & molecular medicine
Dual α-globin and truncated EPO receptor knockin restores hemoglobin production in α-thalassemia-derived red blood cells.
bioRxiv : the preprint server for biology
Reversal of C9orf72 mutation-induced transcriptional dysregulation and pathology in cultured human neurons by allele-specific excision.
Proceedings of the National Academy of Sciences of the United States of America
Give heart cells a beat: An interactive museum exhibit that synchronizes stem cell-derived cardiomyocytes to visitors' heartbeat.
Stem cell reports
Validated assays for the quantification of C9orf72 human pathology.
Scientific reports
Functional analysis of a common BAG3 allele associated with protection from heart failure.
Nature Cardiovascular Research
Evaluation of CRISPR/Cas9 exon-skipping vector for choroideremia using human induced pluripotent stem cell-derived RPE.
The journal of gene medicine
A reference human induced pluripotent stem cell line for large-scale collaborative studies.
Cell stem cell
BRD2 inhibition blocks SARS-CoV-2 infection by reducing transcription of the host cell receptor ACE2.
Nature cell biology
Gain-of-function cardiomyopathic mutations in RBM20 rewire splicing regulation and re-distribute ribonucleoprotein granules within processing bodies.
Nature communications
BRD2 inhibition blocks SARS-CoV-2 infection by reducing transcription of the host cell receptor ACE2.
bioRxiv : the preprint server for biology
Allele-Specific Gene Editing Rescues Pathology in a Human Model of Charcot-Marie-Tooth Disease Type 2E.
Frontiers in cell and developmental biology
Transcription factor overexpression drives reliable differentiation of retinal pigment epithelium from human induced pluripotent stem cells.
Stem cell research
SARS-CoV-2 infection of human iPSC-derived cardiac cells reflects cytopathic features in hearts of patients with COVID-19.
Science translational medicine
SARS-CoV-2 infection of human iPSC-derived cardiac cells predicts novel cytopathic features in hearts of COVID-19 patients.
bioRxiv : the preprint server for biology
Timed inhibition of CDC7 increases CRISPR-Cas9 mediated templated repair.
Nature communications
CRISPR off-target detection with DISCOVER-seq.
Nature protocols
Cardiac Microtissues: Maladaptive Contractility of 3D Human Cardiac Microtissues to Mechanical Nonuniformity (Adv. Healthcare Mater. 8/2020).
Advanced Healthcare Materials
Maladaptive Contractility of 3D Human Cardiac Microtissues to Mechanical Nonuniformity.
Advanced Healthcare Materials
Abstract 934: Physical and Genetic Interaction Analyses in Human Pluripotent Stem Cell-Derived Cardiomyocytes to Study Protein Quality Control Disease in the Heart.
Circulation research
Phenotype-Based High-Throughput Classification of Long QT Syndrome Subtypes Using Human Induced Pluripotent Stem Cells.
Stem cell reports
Mapping cis-regulatory chromatin contacts in neural cells links neuropsychiatric disorder risk variants to target genes.
Nature genetics
A NOVEL ROLE FOR THE FTD-CAUSING PROTEIN PROGRANULIN IN APOPTOSIS SIGNALING.
Alzheimer's & Dementia
Unbiased detection of CRISPR off-targets in vivo using DISCOVER-Seq.
Science (New York, N.Y.)
MESP1 knock-down in human iPSC attenuates early vascular progenitor cell differentiation after completed primitive streak specification.
Developmental biology
Contractile deficits in engineered cardiac microtissues as a result of MYBPC3 deficiency and mechanical overload.
Nature biomedical engineering
Quantitatively characterizing drug-induced arrhythmic contractile motions of human stem cell-derived cardiomyocytes.
Biotechnology and bioengineering
Quantitatively Characterizing Drug-Induced Arrhythmic Contractile Motions of Human Stem Cell-Derived Cardiomyocytes.
Biotechnology and bioengineering
Generation of spatial-patterned early-developing cardiac organoids using human pluripotent stem cells.
Nature protocols
Detection and Quantification of HDR and NHEJ Induced by Genome Editing at Endogenous Gene Loci Using Droplet Digital PCR.
Methods in molecular biology (Clifton, N.J.)
Multi-Imaging Method to Assay the Contractile Mechanical Output of Micropatterned Human iPSC-Derived Cardiac Myocytes.
Circulation research
CRISPRi-based genome-scale identification of functional long noncoding RNA loci in human cells.
Science (New York, N.Y.)
Engineered human pluripotent-stem-cell-derived intestinal tissues with a functional enteric nervous system.
Nature medicine
GENT-49. SYSTEMATIC IDENTIFICATION OF ESSENTIAL LONG NON-CODING RNA GENES IN GLIOBLASTOMA.
Neuro-oncology
BMP-SMAD-ID promotes reprogramming to pluripotency by inhibiting p16/INK4A-dependent senescence.
Proceedings of the National Academy of Sciences of the United States of America
Organs-on-a-chip – Microphysiological platforms as in vitro models of cardiac and adipose tissue.
Toxicology letters
Detecting Single-Nucleotide Substitutions Induced by Genome Editing.
Cold Spring Harbor protocols
Using Digital Polymerase Chain Reaction to Detect Single-Nucleotide Substitutions Induced by Genome Editing.
Cold Spring Harbor protocols
Ligand-binding domains of nuclear receptors facilitate tight control of split CRISPR activity.
Nature communications
Miniaturized iPS-Cell-Derived Cardiac Muscles for Physiologically Relevant Drug Response Analyses.
Scientific reports
Systematic quantification of HDR and NHEJ reveals effects of locus, nuclease, and cell type on genome-editing.
Scientific reports
CRISPR Interference Efficiently Induces Specific and Reversible Gene Silencing in Human iPSCs.
Cell stem cell
Generating trunk neural crest from human pluripotent stem cells.
Scientific reports
Efficient CRISPR/Cas9-Based Genome Engineering in Human Pluripotent Stem Cells.
Current protocols in human genetics
Self-organizing human cardiac microchambers mediated by geometric confinement.
Nature communications
Dysregulation of locus coeruleus development in congenital central hypoventilation syndrome.
Acta neuropathologica
Loss of Gi G-Protein-Coupled Receptor Signaling in Osteoblasts Accelerates Bone Fracture Healing.
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research
Structure-based discovery of NANOG variant with enhanced properties to promote self-renewal and reprogramming of pluripotent stem cells.
Proceedings of the National Academy of Sciences of the United States of America
A non-invasive platform for functional characterization of stem-cell-derived cardiomyocytes with applications in cardiotoxicity testing.
Stem cell reports
Human iPSC-based cardiac microphysiological system for drug screening applications.
Scientific reports
Assessing the osteoblast transcriptome in a model of enhanced bone formation due to constitutive Gs-G protein signaling in osteoblasts.
Experimental cell research
Gai/o-coupled receptor signaling restricts pancreatic �-cell expansion.
Proceedings of the National Academy of Sciences of the United States of America
Astrocytic adenosine receptor A2A and Gs-coupled signaling regulate memory.
Nature neuroscience
Automated Video-Based Analysis of Contractility and Calcium Flux in Human-Induced Pluripotent Stem Cell-Derived Cardiomyocytes Cultured over Different Spatial Scales.
Tissue engineering. Part C, Methods
Calcium transients closely reflect prolonged action potentials in iPSC models of inherited cardiac arrhythmia.
Stem cell reports
Synthetic control of mammalian-cell motility by engineering chemotaxis to an orthogonal bioinert chemical signal.
Proceedings of the National Academy of Sciences of the United States of America
Human induced pluripotent stem cell-based microphysiological tissue models of myocardium and liver for drug development.
Stem cell research & therapy
Induced pluripotent stem cells from patients with human fibrodysplasia ossificans progressiva show increased mineralization and cartilage formation.
Orphanet journal of rare diseases
The C-terminus of the long AKAP13 isoform (AKAP-Lbc) is critical for development of compensatory cardiac hypertrophy.
Journal of molecular and cellular cardiology
Sculpting genomes with a hammer and chisel.
Nature methods
A robust method to derive functional neural crest cells from human pluripotent stem cells.
American journal of stem cells
Derivation conditions impact X-inactivation status in female human induced pluripotent stem cells.
Cell stem cell
GO-Elite: a flexible solution for pathway and ontology over-representation.
Bioinformatics (Oxford, England)
WikiPathways: building research communities on biological pathways.
Nucleic acids research
Model for long QT syndrome type 2 using human iPS cells demonstrates arrhythmogenic characteristics in cell culture.
Disease models & mechanisms
Mineral composition is altered by osteoblast expression of an engineered G(s)-coupled receptor.
Calcified tissue international
Blockade of receptor-activated G(i) signaling in osteoblasts in vivo leads to site-specific increases in cortical and cancellous bone formation.
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research
Constitutive Gs activation using a single-construct tetracycline-inducible expression system in embryonic stem cells and mice.
Stem cell research & therapy
Finding the right questions: exploratory pathway analysis to enhance biological discovery in large datasets.
PLoS biology
Stem cell pluripotency: alternative modes of transcription regulation.
Cell cycle (Georgetown, Tex.)
Reporter-based isolation of induced pluripotent stem cell- and embryonic stem cell-derived cardiac progenitors reveals limited gene expression variance.
Circulation research
AltAnalyze and DomainGraph: analyzing and visualizing exon expression data.
Nucleic acids research
Alternative splicing regulates mouse embryonic stem cell pluripotency and differentiation.
Proceedings of the National Academy of Sciences of the United States of America
Gs G protein-coupled receptor signaling in osteoblasts elicits age-dependent effects on bone formation.
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research
Ligand-mediated activation of an engineered gs g protein-coupled receptor in osteoblasts increases trabecular bone formation.
Molecular endocrinology (Baltimore, Md.)
Cloning mice and men: prohibiting the use of iPS cells for human reproductive cloning.
Cell stem cell
The BridgeDb framework: standardized access to gene, protein and metabolite identifier mapping services.
BMC bioinformatics
G(i)-coupled GPCR signaling controls the formation and organization of human pluripotent colonies.
PloS one
Alternative splicing in the differentiation of human embryonic stem cells into cardiac precursors.
PLoS computational biology
AKAP10 (I646V) functional polymorphism predicts heart rate and heart rate variability in apparently healthy, middle-aged European-Americans.
Psychophysiology
Obtaining consent for future research with induced pluripotent cells: opportunities and challenges.
PLoS biology
SNPLogic: an interactive single nucleotide polymorphism selection, annotation, and prioritization system.
Nucleic acids research
Presenting and exploring biological pathways with PathVisio.
BMC bioinformatics
Engineering GPCR signaling pathways with RASSLs.
Nature methods
WikiPathways: pathway editing for the people.
PLoS biology
Marking embryonic stem cells with a 2A self-cleaving peptide: a NKX2-5 emerald GFP BAC reporter.
PloS one
An Introduction to Pathway Analysis with GenMAPP.
NCI Nature Pathway Interaction Database
Comparative analysis of wild-type and cAMP-deathless S49 lymphoma cells identifies genes and pathways of cAMP/PKA-promoted apoptosis.
The FASEB Journal
Osteoblast expression of an engineered Gs-coupled receptor dramatically increases bone mass.
Proceedings of the National Academy of Sciences of the United States of America
Conditional expression of a Gi-coupled receptor in osteoblasts results in trabecular osteopenia.
Endocrinology
Gene expression signatures of cAMP/protein kinase A (PKA)-promoted, mitochondrial-dependent apoptosis. Comparative analysis of wild-type and cAMP-deathless S49 lymphoma cells.
The Journal of biological chemistry
Modeling insertional mutagenesis using gene length and expression in murine embryonic stem cells.
PloS one
GenMAPP 2: new features and resources for pathway analysis.
BMC bioinformatics
Canonical Wnt signaling is a positive regulator of mammalian cardiac progenitors.
Proceedings of the National Academy of Sciences of the United States of America
Interfacing silicon nanowires with mammalian cells.
Journal of the American Chemical Society
Gene-trapped mouse embryonic stem cell-derived cardiac myocytes and human genetics implicate AKAP10 in heart rhythm regulation.
Proceedings of the National Academy of Sciences of the United States of America
New tools to build synthetic hormonal pathways.
Proceedings of the National Academy of Sciences of the United States of America
Development of hydrocephalus in mice expressing the G(i)-coupled GPCR Ro1 RASSL receptor in astrocytes.
The Journal of neuroscience : the official journal of the Society for Neuroscience
mGluR2 acts through inhibitory Galpha subunits to regulate transmission and long-term plasticity at hippocampal mossy fiber-CA3 synapses.
Proceedings of the National Academy of Sciences of the United States of America
A Series of RASSLs Activated by a Single Agonist.
The FASEB Journal
The International Gene Trap Consortium Website: a portal to all publicly available gene trap cell lines in mouse.
Nucleic acids research
Sustained preconditioning induced by cardiac transgenesis with the tetracycline transactivator.
American journal of physiology. Heart and circulatory physiology
Gene expression patterns define key transcriptional events in cell-cycle regulation by cAMP and protein kinase A.
Proceedings of the National Academy of Sciences of the United States of America
Cardiac transgenesis with the tetracycline transactivator changes myocardial function and gene expression.
Physiological genomics
Constitutive activity of the melanocortin-4 receptor is maintained by its N-terminal domain and plays a role in energy homeostasis in humans.
The Journal of clinical investigation
A public gene trap resource for mouse functional genomics.
Nature genetics
Engineering the melanocortin-4 receptor to control G(s) signaling in vivo.
Annals of the New York Academy of Sciences
MAPPFinder: using Gene Ontology and GenMAPP to create a global gene-expression profile from microarray data.
Genome biology
Regression approaches for microarray data analysis.
Journal of computational biology : a journal of computational molecular cell biology
GenMAPP, a new tool for viewing and analyzing microarray data on biological pathways.
Nature genetics
Tools for dissecting signaling pathways in vivo: receptors activated solely by synthetic ligands.
Methods in enzymology
Engineering receptors activated solely by synthetic ligands (RASSLs).
Trends in pharmacological sciences
Abnormal contraction caused by expression of G(i)-coupled receptor in transgenic model of dilated cardiomyopathy.
American journal of physiology. Heart and circulatory physiology
Tetracycline-inducible system for photoreceptor-specific gene expression.
Investigative ophthalmology & visual science
Conditional expression of a Gi-coupled receptor causes ventricular conduction delay and a lethal cardiomyopathy.
Proceedings of the National Academy of Sciences of the United States of America
A critical period of vulnerability to hyperactive Gq signaling revealed by inducible and heart-specific expression of Gq* in transgenic mice.
Journal of cardiac failure
Genomic analysis of an inducible and reversible model of dilated cardiomyopathy.
Journal of cardiac failure
Chimeric G proteins allow a high-throughput signaling assay of Gi-coupled receptors.
Analytical biochemistry
Conditional expression and signaling of a specifically designed Gi-coupled receptor in transgenic mice.
Nature biotechnology
Controlling signaling with a specifically designed Gi-coupled receptor.
Proceedings of the National Academy of Sciences of the United States of America
The N-terminal extension of Galphaq is critical for constraining the selectivity of receptor coupling.
The Journal of biological chemistry
Interactions of muscarinic receptors with the heterotrimeric G proteins Gq and G12: transduction of proliferative signals.
Journal of neurochemistry
Carboxyl-terminal mutations of Gq alpha and Gs alpha that alter the fidelity of receptor activation.
Molecular pharmacology
Molecular mechanisms involved in muscarinic acetylcholine receptor-mediated G protein activation studied by insertion mutagenesis.
The Journal of biological chemistry
Identification of a receptor/G-protein contact site critical for signaling specificity and G-protein activation.
Proceedings of the National Academy of Sciences of the United States of America
Coupling of the alpha 2A-adrenergic receptor to multiple G-proteins. A simple approach for estimating receptor-G-protein coupling efficiency in a transient expression system.
The Journal of biological chemistry
G alpha 13 stimulates Na-H exchange.
The Journal of biological chemistry
Type II adenylylcyclase integrates coincident signals from Gs, Gi, and Gq.
The Journal of biological chemistry
Structural elements of G alpha subunits that interact with G beta gamma, receptors, and effectors.
Cell
Substitution of three amino acids switches receptor specificity of Gq alpha to that of Gi alpha.
Nature
A recombinant calcitonin receptor independently stimulates 3',5'-cyclic adenosine monophosphate and Ca2+/inositol phosphate signaling pathways.
Molecular endocrinology (Baltimore, Md.)
Gz-mediated hormonal inhibition of cyclic AMP accumulation.
Science (New York, N.Y.)
Recombinant Gq alpha. Mutational activation and coupling to receptors and phospholipase C.
The Journal of biological chemistry
Detection of coincident signals by G proteins and adenylyl cyclase.
Cold Spring Harbor symposia on quantitative biology
Stimulation of arachidonic acid release and inhibition of mitogenesis by cloned genes for muscarinic receptor subtypes stably expressed in A9 L cells.
Proceedings of the National Academy of Sciences of the United States of America
Distinct bradykinin receptors mediate stimulation of prostaglandin synthesis by endothelial cells and fibroblasts.
The Journal of pharmacology and experimental therapeutics