Imaging Core

Cutting-edge technology at your fingertips

Core Support

Thanks to the generous gift from the Gordon and Betty Moore Foundation, in 2008 the Eli & Edythe Broad Center of Regeneration Medicine and Stem Cell Research was given the ability to purchase multiple Leica confocal microscopes with the goal of providing cutting edge microscopy to the Broad Center's researchers. Since that time, we have continued to build on this goal by upgrading our original microscopes and adding new resources and for our core users. 

Equipment

  • Leica SP5X inverted, white light confocal microscope with environmental chamber.
    Hourly rate: $87.09
    Location: Pod D
    Objectives: 10x Dry, 20x Dry, 63x water, 63x oil, 100x oil available on request.  WWL and 405 laser.
  • Leica SP5 upright confocal microscope with AOBS and five laser lines from 405-633.
    Hourly rate: $92.62
    Location: Pod C
    Objectives: 10x multi, 20x multi, 40x water, 63x oil, 63x water. 100x oil available on request.
  • Leica SP8X inverted confocal microscope with environmental chamber, argon laser.
    Hourly rate: $72.80
    Location: Pod B
    Objectives: 10x, 20x oil, 25x, 40x oil, 63x, 100x oil available on request.
  • Leica SP5 upright confocal microscope with AOBS and five laser lines from 405-633.
    Hourly rate: $67.49
    Location: Pod A
    Objectives: 10X Dry, 20X Dry, 40X water, 63X water, 63X oil, 100X oil available on request.
  • Keyence B7-X710 automated fluorescence microscope. Does not have environmental chamber.
    Hourly rate: $67.28
    Location: Pod C
    Objectives: 2x,10x, 20x, 40x, 100x. Filters: GFP, TRITC, DAPI, CY5.
  • PC workstation running Imaris software suite versions 7.1-9.0.
    Hourly rate: There is no charge to use this workstation for microscopy core users.
    Location: Pod D
    Objectives: 256GB DDR4 SDRAM, Intel Xeon E5-2697V4 2.3 GHz processor, NVIDIA Auadro P500 graphics card.
    To Reserve: Please contact William if you would like to have access to this workstation.
  • Leica Thunder 3D fluorescence imager with LED light source and environmental chamber. 
    Location: Pod B
    Availability: Will be available to core users starting in late August 2019.
    Hourly rate: TBD
    Objectives: 2.5x, 5x, 10x, 20x, 40x, 63x objectives.

Access & Training

Access to the core can be obtained after attending our hands-on training course held on the 3rd Tuesday of each month at 10:00am in Pod A, room 904D.
Please note: this training covers only the Leica SP5 platform.

Training for our SP8 WLL microscope is held every three to four months during our “Dr. is in” sessions. The sessions are provided by Leica and consist of a one hour talk with lunch provided, followed by a 2-4 hour training depending on group size and experience level. These sessions are a great opportunity to fine tune your microscopy skills with a Leica technical expert.

If you would like to attend the training or one of these sessions please contact our Imaging Core Manager, William Walantus, at [email protected].

Services

The Imaging Core staff are dedicated to providing user-friendly scheduling, accounting, basic training and oversight to the IRM imaging community. The BCMC does not provide in-depth or experiment-specific training or consultation to users. Advanced microscopy training and project specific consultation are available at other locations on campus including:

Scheduling

To schedule time on BCMC microscopes first attend our hands-on training held once per month on the Third Tuesday of the month at 10am in Pod A, room 904D. If you would like to attend the training please contact our Imaging Core Manager, William Walantus at [email protected].

You will also need a MyCORES account to reserve the microscopes.

Contacts

Core Manager
William Walantus
[email protected]
415.476.0847 

Core Supervisor
Georgia Panagiotakos
[email protected]

Associate Director of Finance & Operations
Maribeth Ruperto
[email protected]