Dieter Gruenert, Ph.D.
Dieter Gruenert, Ph.D.
Cell and molecular biology studies that focus on the generation, characterization and directed differentiation of pluripotent stem cells for the study of disease processes and the development of new therapies for inherited diseases (e.g. cystic fibrosis and hemoglobinopathies) and cancer.
Selected Publications
Gruenert DC, Basbaum CB, Welsh MJ, Li M, Finkbeiner WE, Nadel JA. Characterization of human tracheal epithelial cells transformed by an origin-defective simian virus 40. Proc Natl Acad Sci USA 85:5951-5955, 1988.
KunzelmannK, LegendreJ-Y, Kneoll D, Escobar LC, Xu Z, Gruenert DC. Gene targeting of CFTR DNA in CF epithelial cells. Gene Therapy, 3:859-867, 1996
Goncz KK, Prokopishyn NL, Chow BL, Davis BR, Gruenert DC. Application of SFHR to gene therapy of monogenic disorders. Gene Therapy 9:691-694, 2002.
Goncz KK, Colosimo A, Dallapiccola B, Gagne L Hong K, Novelli G, Papahadjopoulos D, Sawa T, Schreier H, Wiener-Kronish J, Xu Z, Gruenert DC. Expression of ∆F508 CFTR in mouse lung after site-specific modification of normal CFTR sequences by SFHR. Gene Therapy 8(12):961-965, 2001.
Colosimo A, Goncz KK, Novelli G, Dallapiccola B, Gruenert DC. Targeted correction of a defective selectable marker gene in human epithelial cells by small DNA fragments. Mol Ther 3(2):178-185, 2001.
Goncz KK, Prokopishyn NL, Abdolmohammadi A, Bedayat B, Maurisse R, Davis BR, Gruenert DC. SFHR-mediated modification of genomic ß-globin sequences in human hematopoietic stem/progenitor cells. Oligonucleotides 16:213-224, 2006.
de Semir D, Maurisse R, Vock E, Gruenert DC. Immortalization strategies for epithelial cells in primary culture. In Biotechnology: Pharmeceutical Aspects; Drug Absorption Studies – In Situ, In Vitro and In Silico Tools, Ed: Ehrhardt C, Kim K-J, Springer, New York, NY, pp616-639 , 2008
Sargent RG, Kim S, Gruenert DC. Oligo/polynucleotide-based Gene Modification: Therapeutic Potential. Oligonucleotides 21:55-75, 2011
