Welcome to the Imaging Core for the Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research at UCSF.
Thanks to a generous gift from the Gordon and Betty Moore Foundation, in 2008 The Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research at UCSF was able to purchase two Leica SP5 confocal microscopes, one Leica SP5 multiphoton microscope, as well as two image rendering computer stations. In order to provide training, schedule, supervise, and maintain this new equipment, the Center has established the Imaging Core.
- Confocal Scanner on Inverted Microscope for general 3D imaging of samples stained with a combination of fluorophores from UV to IR
- Multi-photon system on inverted microscope for long-term living cell and deep tissue imaging. System is equipped with dual scan devices – allowing imaging ranging from 1 line/sec to 16,000 lines/sec
- Volocity Image rendering Mac workstation
- PC workstation running the software suite Imaris. Although the capabilities of Imaris and Volocity have considerable overlap, Imaris is especially suited for processing extremely large data sets. The workstation is running on 16GB of RAM on a 64-bit system, and should be able to handle the kinds of data from high-resolution still image stacks and also long-term time-lapse imaging. One unique feature of Imaris is its “Filament Tracer” module, which accurately skeletonizes filamentous cells and can quantify things like primary, secondary, and tertiary branches and spine length etc.
Access to the core can be obtained after attending our hands on training course conducted on the third Tuesday of the month. If you would like to attend the training please contact our Imaging Core Manager, William Walantus atWalantusW@stemcell.ucsf.edu.
The Imaging Core staff are dedicated to providing user-friendly scheduling, accounting, basic training and oversight to the IRM imaging community while ensuring that users are adequately trained and the microscopes are properly operated and maintained. The Imaging Core does not provide in-depth or experiment-specific training or consultation to users. Advanced microscopy training and project specific consultation are available at other locations on the Parnassus campus including:
- Diabetes Center Microscopy Core
- Biological Imaging Development Center
- Cancer Center Laboratory for Cell Analysis
The Laboratory for Cell Analysis has trained personnel available specifically for direct assistance with image collection. Please check the above websites for access and recharge information. The Diabetes Center also provides a free monthly 75-minute introductory lecture that any user is welcome, and encouraged, to attend.
To schedule usage time on any of the equipment in the Imaging Core, please access our online Imaging Core Scheduler.